Original Article

Year : 2015

Volume : Volume 5

Issue : Issue 1

A Comparative Analysis of Antimicrobial Efficacy on Enterococcus Faecalis and Candida Albicans using Various Irrigants along with Laser and Ultrasonic Device: An in Vitro Study

Dr. S Sudhakar Naidu

Address for correspondence :
Dr. S Sudhakar Naidu
sudhakarnaidu19@gmail.com

Abstract


Aim: To evaluate the efficacy of different concentration of different irrigants and their antimicrobial efficacy. Materials and Methods: 240 single rooted human permanent teeth with straight canals were collected and decoronated upto 15 mm from the apex. Cleaning and shaping of all teeth was done using step down method. The diameter of the orifice opening of all teeth was standardized. The specimens were infected with the microorganisms (E.faecalis and C.albicans). Later teeth were randomly grouped and irrigated with their respective irrigating solutions (Sodium hypochlorite and Chlorhexidine) and agitation devices (Laser and ultrasonic). After irrigation the samples were processed and carried to the microbiological lab and cultivated for viable organisms where colony forming units were observed indicating the presence of organisms. Results: Kruskal-Wallis one-way ANOVA test and Mann-Whitney U-test was done to statistically analyze antimicrobial efficacy of two irrigants at various concentrations. There was significant difference among the groups. Conclusion: 2 % Sodium hypochlorite agitated with laser showed better antimicrobial efficacy. Least antimicrobial efficacy was seen with 0.5 % Chlorhexidine without any agitation.

Keywords: Sodium hypochlorite, Chlorhexidine, Laser, Ultrasonic, E.faecalis, C.albicans.