Year : 2018

Volume : Volume 9

Issue : Issue 3s

Address for correspondence :
Dr. Harbinder Singh
jolly17107@yahoo.co.in

Abstract

Background: To compare, in vitro, the efficacy of two different dyes used for microleakage analysis of an endodontic sealer based on zinc oxide & eugenol. The two dyes used were Hematoxyline and Propiodium Iodide.  Methodology:  30 single rooted human teeth were selected & cleaned of all debris. The coronal part of each tooth was removed with carborundum disks. They were then divided into 2 different groups, namely, Hematoxyline dye group (15 teeth) and Propiodium Iodide dye group (15 teeth). Each group was further divided into 3 sub-groups: Sub-group A - Negative control (non-instrumented), Sub-group B - Positive control (instrumented), Sub-group C - Experimental (obturated). The specimens of each group were immersed in their respective different dyes. After washing the samples, they were longitudinally sectioned, through the centre of the root, at the cemento-enamel junction. Microleakage was then assessed using a phase contrast and fluorescent microscope.   Results: The mean leakage values, as determined by Propiodium Iodide dye penetration were significantly greater than that quantified by Hematoxyline dye penetration. Conclusion: Within the limits of this in vitro study it can be concluded that the staining provided by Propiodium Iodide dye was significantly greater than the staining provided by Hematoxyline dye.

Keywords: Propiodium Iodide dye, Hematoxyline dye, Lateral condensation, Endoflux, Dye penetration, Microleakage,  Fluorescent microscopy.