Original Article

Year : 2019

Volume : Volume 10

Issue : Issue 1s

Comparative Evaluation of Antimicrobial Efficacy of Phytomedicinal Extracts and Chemical Irrigant against Enterococcus faecalis and Candida albicans: In vitro study

Dr. Kapil D. Wahane.

Address for correspondence :
Dr. Kapil D. Wahane.
drsweetylohani@gmail.com

Abstract


Background:  To compare and evaluate the antimicrobial activity of phytomedicinal extracts and chemical irrigant against E.faecalis and C. albicans using disc diffusion method.  Materials and Methods: For the study, microbial strain of Enterococcus faecalis and Candida albicans were used. Four experimental groups were divided. These were as follow- Group I- Garlic Extracts, Group II- Ginger extracts, Group III- 5.25% Sodium Hypochlorite and Group IV- 2 % Chlorohexidine Gluconate. For the agar disc diffusion test, Petri plates of Muller Hinton agar were inoculated with 0.1 ml of the microbial suspensions. Subsequently, the paper discs of four groups were placed on the Muller Hinton agar plate. After 24 hours, the diameter of inhibition zone was measured around the papers discs containing the substances. Results: Statistically significant difference (p<0.05) was found between the groups against E. faecalis and C. albicans. Order of antimicrobial activity is Garlic >2 % Chlorohexidine gluconate > 5.25% Sodium Hypochlorite> Ginger. Conclusions: Garlic extract is advantageous in cases of secondary endodontic infections which are dominated by organisms like E. faecalis and C. albicans.

Keywords: Enterococcus faecalis, Candida albicans, disc diffusion, ginger extract, garlic extract.