Year : 2024

Volume : Volume 15

Issue : Issue 5

Abstract

Background: The objective of this study was to compare the biocompatibility of titanium-based and stainless steel-based orthodontic Mini screws/ temporary anchorage devices. Materials and Methods: A cell culture system using commercially available human osteoblast cells was utilized. Mini screws/ temporary anchorage devices obtained from Revera Orthodontics were immersed in a culture medium for 24 hours. The surface area to volume character of the eluates produced by this method was within the suggested ISO range. Human osteoblasts were exposed to a Mini screw/temporary anchorage device conditioned culture medium. Biocompatibility and cytotoxicity were measured using a colourimetric 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: MTT analysis, used to quantify viable cells, demonstrated that 24-hour conditioned medium significantly affected cell viability. Conclusion: When observed for 24 hours, titanium-based implants showed less cell death than stainless steel implants. The long-term effect of implants on cell viability needs to be studied further.