Original Article

Year : 2021

Volume : Volume 12

Issue : Issue 6

Enamel Remineralization System: Front Line in Caries Management

: Dr. Shivani Soni,

Address for correspondence :
: Dr. Shivani Soni,
shivanisoni1992.ss@gmail.com

Abstract


Background: The initial carious lesions are the professed “white spot” lesions, which indicatethat there is a subsurface area with most of the mineral loss underneath a relatively intact enamel surface. With a clear understanding regarding the demineralization-remineralization theory of caries initiation, importace in present-day dentistry is on remineralization of carious lesion. Thus implying that remineralization
could occur in clinically detectable incipient lesions. Therefore, the aim of the present study was to compare and evaluate the effectivenessof the remineralization potential of Nanohydroxyapatite and Casein Phosphopeptide-Amorphous Calcium Phosphate (CPP-ACP) toothpastes on enamel. Materials and Methods: Freshly extracted caries-free intact human primary molar were used for this study. Forty-eight enamel
blocks (4 mm × 4 mm) were prepared from the smooth surfaces. All the specimens were immersed in the demineralizing solution for a period of 6 days. These specimens were randomly divided into two groups of 24 each. In Group-I specimen were treated with Nano-hydroxyapatite (n-HAP) toothpaste and in Group II specimen by CPP-ACP mousse. Specimens were manually brushed, twice daily for 3 min and were stored
in the saliva solution. After 2 weeks, 12 samples of each groups were sent to the laboratory for evaluation of enamel surface morphologies, using scanning electron microscope (SEM) and for evaluation of Calcium and Phosphate mineral component, SEM energy dispersive X-ray (SEM- EDX). Results: SEM: n-HAP Toothpaste Group demonstrated more remineralized structure than CPP-ACP Group. The surface of the
teeth treated with n-HAP displayed nucleation of apatitic crystals. SEM-EDX: Mean Ca (4.084 ± 1.603) and P (2.787 ± 1.699) was higher in n-HAP Toothpaste Group than CPP-ACP Group. This was statistically significant difference. Conclusion: Based on the data obtained from the present study, it was concluded that although both CPP-ACP and n-HAP demonstrated remineralization potential, the samples treated with n-HAP illustrated statistically significant higher remineralization.
Keywords: Calcium, Casein Phosphopeptide-Amorphous Calcium Phosphate, Demineralization, Nano-hydroxyapatite, Phosphate, Primary
Teeth, Remineralization, Scanning electron microscope, Scanning electron microscope - energy dispersive X-ray