Year : 2019

Volume : Volume 10

Issue : Issue 1

Address for correspondence :
Dr. Randhir Kumar.
drrandhirmds@gmail.com

Abstract

Aim:  To establish salivary Lactate dehydrogenase as biomarkers for assessing the malignant potential  in leukoplakia and oral squamous cell carcinoma patient in order to improve the prognosis and outcome of the disease. Materials and Methods:  Total 90 subjects and their saliva samples were taken to estimate lactate dehydrogenase level in oral squamous cell carcinoma, leukoplakia and healthy subject. 2 ml of unstimulated saliva was collected from the patients by spit method in a wide mouthed sterile container. It was then immediately centrifuged at 2500 rpm for 15 minutes to remove squamous cell and cell debris. The resulting supernatant was then separated into 1 ml aliquots and subjected for further biochemical assay analysis using standard kit method. The samples will then be diluted in 1:1 ratio with saline and assayed using standard kit and measured using semi auto analyzer. The salivary LDH concentrations were expressed in terms of IU/L. Results: A highly significant association of lactate dehydrogenase was observed in leukoplakia and squamous cell carcinoma patient. The present study also showed correlation between tumor differentiation and the level of salivary LDH in oral squamous cell carcinoma patient and different grade of dysplasia. Conclusion: Lactate dehydrogenase are sensitive markers for the detection of pre cancer and cancer hence helpful in early detection of oral carcinoma. Statistical analysis also proves that lactate dehydrogenase levels are consistently higher in oral pre cancer and cancer hence it could be more reliable marker in detection of oral cancer.

Keywords: Leukoplakia, Oral Squamous Cell Carcinoma, Saliva, Lactate dehydrogenase.