Year : 2015

Volume : Volume 5

Issue : Issue 3

Address for correspondence :
Rethi Mahesh
dr.maheshj@yahoo.co.in

Abstract

Aim: To evaluate and compare in-vitro the antibacterial activity of four endodontic sealers (Sealapex (Calcium hydroxide sealer), Tubliseal (Zinc oxide eugenol sealer), RoekoSeal (Polydimethyl siloxane based sealers), and Endo Rez (Urethane dimethacrylate resin based sealer) Enterococcus faecalis by a direct contact test over a period of time.  Materials and Methodology: The direct contact test was performed based on turbidometric determination of bacterial growth in 96-well microtiter plates. The kinetics of the outgrowth in each well was monitored at 600 nm at 370C. The bacterial outgrowth was monitored both in the presence (A wells) and in the absence of the tested material (B wells). The recordings were based on the reading of the transmittance values in the spectrophotometer. Higher the transmittance value, the higher was the antimicrobial activity (i.e. less microbial growth).The microbial growth was recorded every 1 hour using a spectrophotometer for 7 hours at time intervals of 20mts, 1 day and 7 days.  Results: results are subjected to statistical analysis by Kruskal Wallis One way Anova and Mann –Whitney ‘U’ test.  Conclusion: Under the limitations of this study endodontic root canal sealers had different inhibitory effects on Enterococcus faecalis during the growth period and Zinc oxide Eugenol based sealer (Tubli-Seal EWT) was the most effective and Urethane dimethacrylate resin based sealer (EndoRez) was the least effective against Enterococcus faecalis..

Keywords: Enterococcus faecalis, root canal, sealers, antibacterial, endodontic,direct contact test.