Year : 2015

Volume : Volume 5

Issue : Issue 1

Address for correspondence :
Dr. Vandita Mishra
vandita5jan@gmail.com

Abstract

Aim: Our aim was thus to identify, quantify and compare the levels of S100 proteins in the state of health and in individuals with chronic and aggressive periodontitis.  Methods: This was a single center, cross-sectional study of 4 months duration. A total of 30 subjects were included in the study 10 each with a healthy periodontium and chronic periodontits and 10 with aggressive periodontal disease. 5ml of resting saliva was collected from all the individuals and the samples were immediately sent to the laboratory of the biochemistry department of the institute. The samples were subjected to one dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis to identify and quantify the total S100 proteins.  Results: Protein bands corresponding to lower molecular weight proteins were seen in the aggressive periodontitis individuals. In both healthy subjects and in the chronic periodontitis group these lower molecular weight bands were not identified.  Conclusion: Lower molecular weight proteins were identified in aggressive periodontitis individuals only which may be correlated to the presence of neutrophilic abnormalities in individuals with this disease.

Keywords: Periodontitis, Saliva, Aggressive periodontitis, S100 protein family.