Original Article

Year : 2024

Volume : Volume 15

Issue : Issue 2

Quantification of Calcium Ions in Chitosan, Smear Clear and Citric Acid after Root Canal Irrigation – An In Vitro Atomic Absorption Spectrophotometry Study

Dr. P. Dhanalakshmi

Address for correspondence :
Dr. P. Dhanalakshmi
dhanalakshmi.sdm@gmail.com

Abstract


Background: The use of rotary or hand instruments during endodontic cleaning and shaping procedures forms smear layer inevitably. As the smear layer harbors microorganism and acts as a nidus of infection, the removal of SL provides a sterile environment in the root canal system and enhances the adaptation of sealer to the root canal. Objectives: This study was designed to analyze the calcium ion expelled, resulting from final irrigation with Smear clear, 0.2% Chitosan, 10% citric acid, and de-ionized water (control). Materials and Methods: Sixty human premolars were used in this study and biomechanical preparation was done. During instrumentation, 3% sodium hypochlorite was used and according to the irrigant used finally, grouping of the samples was done with 15 teeth in each group. Group 1 - Smear Clear (SybronEndo, Orange, CA, USA) - 17% ethylenediaminetetraacetic acid solution with cetrimide, polyoxyethylene and iso-octylcyclohexyl ether, Group 2 - 0.2% Chitosan, Group 3 - 10% citric acid, Group 4 - de-ionized water (Control). After final irrigation, these solutions were collected in a falcon tube and subjected to flame atomic absorption spectrometric study. Statistical analysis was carried out using one-way ANOVA and Tukey test. Results: The mean concentration of calcium ions of 1.9211 ± 0.4094 was noted with Smear clear and a value of 1.7891 ± 0.2837 observed with 0.2% chitosan. Statistically, these two groups did not differ. 10% citric acid eluted the least calcium ion. No calcium ion was detected in the control group. Conclusion: 0.2% chitosan is as potential as smear clear in removing the calcium ions and smear layer from root dentin.