Year : 2019

Volume : Volume 10

Issue : Issue 1s

Abstract

Background:  Diabetes mellitus is one of the major endocrinal disorder characterized by hyperglycemia. Purpose of this study was to analyze the glycogen accumulation in the exfoliated cells of oral mucosa as a diagnostic adjunct in diabetes mellitus.  Materials and Methods:  Twenty diabetic patients (study group) and twenty control patients were selected. Three smears were prepared from each patient, stained with hematoxylin and eosin stain, periodic acid Schiff (PAS) stain and PAS-Diastase (PAS-D) stain in both groups. Because of digestion of glycogen by diastase enzyme, magenta colored PAS positive cells were stained lighter with PAS- D stain.  Result:  Results were statistically analyzed and showed that mean and standard deviation values of RBS were found to be higher in study group than the control group and the data was statistically significant. Also the total number of PAS positive cells were more in study group. The study group showed nuclear changes such as enucleation and binucleation in H & E stained slides.  Conclusion: Microscopic examination shows presence of glycogen in buccal exfoliated cells in diabetic patients that can be used as diagnostic tool but need further extensive study.

Keywords: Diabetes mellitus, PAS- D stain, exfoliated cells.